microwave with lcd screen free sample
Short turnaround times as well as reliable good section and image quality are important criteria when rapid embedding protocols are considered. Comparing the samples processed according to a microwave-assisted versus a conventional protocol, we obtained very satisfying results with the microwave bench “oven”. The experienced reduction in sample processing time from days to hours with no compromised ultrastructural preservation of selected “difficult” specimens is in agreement with other workers using this technology. Microwave processing time of 3–4 h (Nordhausen and Barr, 2001, Gerrity and Forbes, 2003, Laboux et al., 2004) and short as 2 h (Giberson et al., 2003) were reported from EM laboratories with similar sample spectrum and workload as those of the authors.
The microwave-assisted processed samples were comparable or superior in section and image quality to the conventionally processed samples and compliant to published standards (Morioka et al., 1992, Miller, 2003, Peiris et al., 2003, Buchanan, 2004, Celandroni et al., 2004, Franzen et al., 2005, Larsson, 2005). The completely microwave-assisted processed parenchymal tissue of liver, used in our comparative approach as a substitute for other pathological solid tissues, revealed good results as shown in Fig. 12 in samples not exceeding 1-mm edge length. In the centre of the block where larger samples had been taken, there was a loss of ultrastructural preservation, and artefacts were seen such as have been described previously by Wild et al. (1989).
This phenomenon reflects the continuing discussion about the probably “dichotomous” nature of the non-ionizing microwave radiation in respect to the “heat” and the “non-thermal” or direct microwave effect (Galvez et al., 2004). Despite the complex aspects influencing the aldehyde fixation process of tissues (Fox et al., 1985, Leong and Sormunen, 1998, Izumi et al., 2000, Werner et al., 2000, Giberson and Elliott, 2001), recent observations tend to support the existence of the direct mechanism of microwave energy action (Kok and Boon, 2003, Leria et al., 2004, Wendt et al., 2004, De la Hoz et al., 2005, Gaber et al., 2005). This needs to be considered in the microwave-assisted tissue processing because also our results suggest, that an “overheating” inside the solid tissue samples can adversely affect tissue ultrastructure. Therefore, further development work should include efforts to reduce the critical “thermal” effect and increase direct “non-thermal” microwave action during tissue fixation (Galvez et al., 2004, Leria et al., 2004).
The REM Milestone microwave device is run with the sample baskets that are used also in the widely applied automated LYNX-processor for routine conventional tissue embedding. We observed that this shared equipment is a great benefit for the EM laboratory, e.g., a sample received late afternoon can be rapidly fixed in the microwave (e.g., 10–20 min in place of at least 2 h at room temperature) and added (without basket change) to other tissues which are to be processed automatically in the LYNX overnight anyway, and if urgent, rapidly polymerized in the microwave in 1 h 45 min the next morning (instead of 48 h). This option generates more flexibility of the laboratory workflow in handling clinical urgent samples and improves the economic employment of the available manpower. Thus, a “same-day” electron microscopy diagnosis for cells and tissues becomes reality.
The results obtained with the shortened protocol appear especially promising. As judged from sectioning properties and section quality, the samples were uniformly polymerized, i.e., comparable to the results with the longer microwave protocol. The observed denser appearance of cell cytoplasm could be explained by less extraction of cell material during the shorter course of the complete procedure. Therefore, in view of the acceptable results with the approximately 3.5 h protocol, attempts to cut down further the preparation schedule appear worthwhile.
Clearly, urgent samples reaching the diagnostic laboratory at unpredictable daytime need to be examined immediately in potential bioterrorism scenario or in case of an emerging agent disease (Lane et al., 2001, Morens et al., 2004). The microwave-assisted sample preparation enables EM to evaluate also thin sections for rapid diagnosis. Basing on our experience, the subsequent section ultramicrotomy, staining and examination in the EM can be completed in about 2 h. In consequence, using the potential of accelerated section preparation, EM-diagnosis from biopsy material can be rendered on the same workday. Thus, both negative staining of small particulate suspensions – from the smallest viruses up to bacteria, spores and parasites – as well as tissues and diagnostic cell cultures can be scrutinized by the “open view” of EM (Hazelton and Gelderblom, 2003) as part of the “frontline” diagnostic methods guiding further measures, e.g., containment and therapeutic activities. In combination with digital image acquisition and telemicroscopy networking tools (Schroeder et al., 2001) crucial or interesting findings could be consulted or shared worldwide in very short time.
Microwaves can do a lot more than just heat cold coffee and frozen dinners. In fact, the additional cooking capability of microwaves (from convection cooking to steaming) has made them more essential than ever, especially if you’re not a planner and need to defrost a piece of meat to cook for dinner.
While countertop microwaves are the most common, an over-the-range (OTR) model can round out your kitchen suite, especially if you prefer appliances that are all from one brand, with matching handles and other details. No matter which type you choose, you’ll want a microwave that measures up in our member surveys for predicted reliability and owner satisfaction.
Once you make the investment, use your microwave with care so that it will last. To extend its life, experts say to regularly clean up messes as soon as they happen and avoid putting metal utensils and dishes inside your microwave, along with six other tips. When used properly, you shouldn’t have to replace a microwave more than once every 10 years, which is how long manufacturers say microwaves should last.
A countertop microwave is a key appliance for any starter kitchen, such as in a first home or an off-campus apartment. And you don’t have to spend a lot to get top performance, as evinced by this selection of countertop microwaves for $150 or less. An OTR microwave might be a good choice if you have limited counter space or prefer a built-in appliance. It’s pricier, though, with costs starting at $200 or more. Pricier still is the built-in microwave, which can be built into the wall or surrounded by cabinetry. Though a built-in microwave is a great option if you want a more elevated-looking kitchen, they can cost much more than other types, with prices ranging from around $300 to upward of $1,400.
At Consumer Reports, we test microwaves in every price range and buy every model we test. Our ratings include small, midsized, and large countertop microwaves; OTR microwaves; and a few built-in models that sleekly integrate into your cabinetry.
Here’s a GE microwave schematic. There’s a lot of typical microwave stuff going on here, but we’re going to focus in on one component today: the monitor switch.
The purpose of the monitor switch is to act as a safety to prevent the magnetron from being energized while the door is open. The door latch and switches in microwaves are designed so that, if everything is working correctly, the monitor switch should never be closed at the same time as the interlock switches. In fact, the only situation in which it would be possible for the monitor switch to be closed at the same time as the door switches is if there’s been a mechanical failure of the door latch/switches.
Well, that does look like it would be a problem! If that monitor switch is closed during operation, it creates a dead short — and that’s by design! The amps in that circuit will immediately jump far above the rated spec on the microwave’s fuse, which will go open and kill the whole unit. A simple, but effective way of making sure the magnetron can never run with the door open.
Want to learn more about microwaves, their technology, and how to troubleshoot them? Click below to check out our online training course on cooking appliances right here at the Master Samurai Tech Academy.